Free Masonry in Marceline dates from October 17, 1889, when the Grand Lodge of the State of Missouri granted a Charter to Marceline Lodge Number 481 authorizing them to meet and work with Milton Helwig, Worshipful Master; I. Jeff Buster, Senior Warden; and John F. Eyler, Junior Warden. The former Masonic Building was located where the present Masonic Temple now stands. The present lodge was completed and dedicated in 1923. Marceline Chapter No. 118 Royal Arch Masons was chartered April 26, 1900. Havre De Grace Commandery No. 63 was chartered May 29, 1909. Following are the names of the Past Masters of Marceline Lodge No. 481 and the years in which they served.
- 1889-1890, M. Helwig
- I. Jeff Buster
- M. Helwig
- W. E. Taylor
- D. L. Batterton
- 1895-1898, George T. Lyda
- T. B. Sloey
- George T. Lyda
- T. B. Sloey
- M. H. Washburn
- Geo. T. Lyda
- 1904-1905, M. Helwig
- B. B. Putman
- O. G. Jordon
- 1908-1909, E.W. Taylor
- C. R. Lodge
- E. H. Banner
- L. A. Mearman
- C.U.Murray
- A. L. Crabb
- B. A. Anderson
- G. W. Early
- F. L. Snider
- W. E. Parks
- E.W. Taylor
- 1919-1920, Chas. Neason
- A. S. Bunch
- H. L. Miller
- P. L. Patrick
- C. M. Hill
- -F. W. McVay
- M.H. Karns
- H.D. Foster
- E.W. Taylor
- H. R. Mason
- R. B. Murray
- F. L. Haddock
- R. W. Wall
- C. W. Rogers
- F. H. Schutte
- H. E. Mason
- H. D. Foster
- J. D. Rusk
- E. K. Taylor
- W. R. Stonger
- A. D. Bunch
- A. F. O’Neil
- F. C. Houser
- F. H. Schutte
- J. J. Cheesman
- J.C. Dieterich
- C. A. McAfee
- C.R.Machen
- A.M. Willian
- H.V.Boddy
- C. A. McAfee
- F.C. Earl
- A.T. Akers
- W. H. Tucker
- R.W. Stauffer
- E.C. Carlson
- E.D.Walsworth
- Earl Doss
- W. H. Zink
- Earl Doss
- Max Snider
- 1961-1962, W. H. Riley